吴青海¹，三 月²，萨初拉¹，郭 煜¹，王力伟¹，王莉梅¹，杨 静¹，其布日^1*

（1.内蒙古自治区农牧业科学院生物技术研究所，呼和浩特010031；2.内蒙古商贸职业学院餐饮食品系，呼和浩特010070）

摘要：该文探究传统发酵乳源短乳杆菌（Lactobacillus brevis）HQ1-1的基因组基本特征和糖代谢表型。经PacBio平台对短乳杆菌HQ1-1进行全基因组测序，并对基因组进行功能分类和注释。结果显示，短乳杆菌HQ1-1包含1条长度为2376708bp的环状染色体和7个大小不同的质粒DNA，编码2268个基因。通过KEGG数据库注释到参与代谢过程的基因有860个，其中，碳水化合物代谢相关基因的数量为142个，占比为16.51%。进一步用CAZy数据库注释到了糖苷水解酶类基因114个，占碳水化合物相关基因总数的45.24%。通过Biolog微阵列表型芯片分析发现，短乳杆菌HQ1-1对L-阿拉伯糖、D-木糖、D-核糖、L-来苏糖等碳源的利用效率相对更高。因此，发酵乳源短乳杆菌HQ1-1基因组中包含碳水化合物代谢相关基因的数量较多，能够利用多种碳源。该文全面阐述了短乳杆菌HQ1-1基因组特征和代谢表型，为今后开发功能性发酵食品提供新型乳酸菌发酵剂

关键词：传统发酵乳；短乳杆菌HQ1-1；全基因组测序；功能基因；糖代谢表型

中图分类号：TS252.1 文献标识码：A 文章编号：1674-506X（2024）06-0001-0008

Lactobacillus brevis HQ1-1 from Traditional Fermented Milk Source and Analysis of Its Genomic Characteristics and Glycometabolic Phenotype

WU Qinghai¹，SAN Yue²，SA Chula¹，GUO Yu¹，WANG Liwei¹，WANG Limei¹，YANG Jing¹，QI Buri^1*

（1.Biotechnology Institute，Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences，Hohhot 010031，China；2.Department of Catering and Food，Inner Mongolia Business and Trade Vocational College，Hohhot 010070，China）

Abstract: In this paper, the basic characteristics of the genome and glycometabolic phenotype of Lactobacillus brevis HQ1-1 from traditional fermented milk were explored. The whole genome sequencing of Lactobacillus brevis HQ1-1 was conducted by PacBio platform, and the functional classification and annotation of the genome were performed. The results showed that Lactobacillus brevis HQ1-1 carried a circular chromosome with a length of 2 376 708 bp and 7 plasmids DNA of different sizes, encoding 2 268 genes. There were 860 genes annotated by KEGG database involved in metabolic process, among them 142 genes were related to carbohydrate metabolism, accounting for 16.51%. Further, 114 glycoside hydrolases genes were annotated using CAZy database, accounting for 45.24% of the total number of carbohydrate-related genes. According to Biolog microarray phenotype chip analysis, Lactobacillus brevis HQ1-1 was found to have relatively higher efficiency of utilizing carbon sources like L-arabinose, D-xylose, D-ribose, L-lyxose. Therefore, the genome of Lactobacillus brevis HQ1-1 contains a large number of genes related to carbohydrate metabolism, possessing utilization capacity for various carbon source. The paper comprehensively elaborates the genome characteristics and metabolic phenotypes of Lactobacillus brevis HQ1-1, and provides novel lactic acid bacteria starter for the development of functional fermented food in the future.

Keywords：traditional fermented milk；Lactobacillus brevis HQ1-1；whole genome sequencing；functional gene；glycometabolic phenotype

doi：10.3969/j.issn.1674-506X.2024.06-001